GCN4-derived expression of heterologous coding sequences

摘要

The invention provides an expression cassette comprising: a regulatory element comprising the nucleotide sequence of the 5'UTR of the gene encoding the GCN4 transcription factor or a functional derivative or fragment thereof; a promoter operably linked upstream to said regulatory element; a heterologous coding sequence downstream to said regulatory element; and a termination signal operably linked downstream to said heterologous sequence; Optionally, the expression cassette further comprises an operably linked selectable marker. The expression system according to this invention provides high level expression of heterologous proteins or peptides which can be regulated at the translational level as well as at the transcriptional level.