| 摘要 |
The present invention deals with a method for the generation of a dynamic combinatorial library of ligands for a given target, capable of binding at least two functionalities, which method comprises the following steps: <v) selecting a plurality of functionalities which may bind to the target; <vi) selecting a set of n molecules carrying said functionalities, which molecules are capable of reversibly associating with each other under formation of an entity, n being an integer ≥ 1; <vii) mixing together said set of said n molecules; <viii) subjecting the mixture to conditions allowing a reversible bond formation and cleavage under formation of entities carrying different combinations of functionalities, until equilibrium is reached; <v) optionally transforming the said entities into ligands; <vi) repeating steps (ii) to (v) n-times, each time a different molecule of said set of n molecules being omitted; <vii) assessing the ability of each of the mixtures obtained to bind to a given target. |
