PHOSPHOLIPASE A1 MUTANT HAVING THERMOSTABILITY AND PREPARATION METHOD THEREOF

摘要

PURPOSE: Provided are phospholipase TA3 and TA13 which are mutants of phospholipase A1 decomposing or synthesizing phospholipid and has thermostability. Also, provided are their coding genes, a recombinant vector containing the genes and a microorganism transformed with the vector. Further provided is a manufacturing method of phospholipase A1 mutant therewith. CONSTITUTION: The phospholipase TA3 and TA13 are derived from Serratia sp. strain MK1, and have nucleotide sequences of the SEQ ID NO:4 and SEQ ID NO:5 respectively and amino acid sequences of SEQ ID NO:6 and SEQ ID NO:7 respectively. The preparation comprises culturing E. coli transformants (Escherichia coliXL-Blue/pTA3, KCTC-18037P, Escherichia coliXL-Blue/pTA13, KCTC-18038P) transformed with vectors, pTA3 and pTA13, containing mutant genes of phospholipase A1 derived from Serratia sp.; separating the above phospholipase A1 mutants from the culture medium and purifying them.