发明名称 |
Apparatus for quantitative analysis of a nucleic acid amplification reaction |
摘要 |
An apparatus for determining a threshold value (e.g., a threshold cycle number or a time value) in a nucleic acid amplification reaction comprises a detection mechanism for measuring, at a plurality of different times during the amplification reaction, at least one signal whose intensity is related to the quantity of a nucleic acid sequence being amplified in the reaction. The apparatus also includes a controller in communication with the detection mechanism. The controller is programmed to perform the steps of deriving a growth curve from the measurements of the signal; calculating a derivative of the growth curve; identifying a characteristic of the derivative; and determining a threshold value associated with the characteristic of the derivative. Embodiments of an apparatus for determining a starting quantity of a nucleic acid sequence in a test sample are also provided.
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申请公布号 |
US2002034745(A1) |
申请公布日期 |
2002.03.21 |
申请号 |
US20010808674 |
申请日期 |
2001.03.14 |
申请人 |
CEPHEID |
发明人 |
MCMILLAN WILLIAM A.;CHRISTEL LEE A.;BORKHOLDER DAVID A.;YOUNG STEVEN J. |
分类号 |
B01L7/00;C07H21/02;C07H21/04;C12M1/00;C12M1/34;C12N9/98;C12P19/34;C12Q1/00;C12Q1/68;G01N21/27;G01N21/64;G01N33/48;G01N33/50;G01N35/00;G06F19/00;(IPC1-7):C12Q1/68 |
主分类号 |
B01L7/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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