发明名称 |
DIRECTED EVOLUTION METHOD |
摘要 |
We describe a method of selecting an enzyme having replicase activity, the method comprising the steps of: (a) providing a pool of nucleic acids comprising members each encoding a replicase or a variant of the replicase; (b) subdividing the pool of nucleic acids into compartments, such that each compartment comprises a nucleic acid member of the pool together with the replicase or variant encoded by the nucleic acid member; (c) allowing nucleic acid replication to occur; and (d) detecting amplification of the nucleic acid member by the replicase. Methods for selecting agents capable of modulating replicase activity, and for selecting interacting polypeptides are also disclosed. |
申请公布号 |
WO0222869(A2) |
申请公布日期 |
2002.03.21 |
申请号 |
WO2001GB04108 |
申请日期 |
2001.09.13 |
申请人 |
MEDICAL RESEARCH COUNCIL;HOLLIGER, PHILIPP;GHADESSY, FARID |
发明人 |
HOLLIGER, PHILIPP;GHADESSY, FARID |
分类号 |
G01N33/53;C12N9/10;C12N9/12;C12N15/09;C12N15/10;C12N15/54;C12Q1/02;C12Q1/48;C12Q1/68;G01N33/15;G01N33/566 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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