发明名称 |
Method for detecting PSA and its molecular forms using thiophilic gel |
摘要 |
<p>A method for capturing PSA and its molecular forms that may be in a fluid biological material including the steps of: preparing a chromatographic column by placing a thiophilic gel in a column where the thiophilic gel is formed from a water insoluble polymer where the surface of the gel is provided with thiophilic moieties that bind PSA in the presence of an adsorption liquid but that will release PSA upon elution with an eluting liquid, said thiophilic moieties comprising a two part structure wherein one part can be characterized as a hydrophilic electron acceptor and the other part is sulfur which acts as an electron donor; selecting a sample of a fluid biological material to be tested for PSA and its complexes; introducing the sample into the column; eluting the sample through the column; rinsing the column with adsorption liquid to remove materials that are unbound to the thiophilic gel; and capturing PSA and its complexes in eluted column fractions.</p> |
申请公布号 |
EP1189063(A1) |
申请公布日期 |
2002.03.20 |
申请号 |
EP20010116967 |
申请日期 |
2001.07.12 |
申请人 |
HEALTH RESEARCH, INC. |
发明人 |
CHADHA, KAILASH C.;SULKOWSKI, EUGENE;KAWINSKI, ELZBIETA |
分类号 |
G01N33/50;B01J20/281;B01J20/285;C07K1/16;C07K14/47;G01N30/34;G01N30/62;G01N30/88;G01N33/574;G01N33/68;(IPC1-7):G01N33/574 |
主分类号 |
G01N33/50 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|