发明名称 |
Method for detecting microorganisms by fluorescent staining using hydrostatic pressure |
摘要 |
A method for detecting microorganisms present in a sample comprises the steps of: applying a non-lethal hydrostatic pressure to a sample containing microorganisms; and staining the microorganisms with a fluorescent dye. The method permits a significant increase in the uptake of a fluorescent substance by applying, to a sample, a desired non-lethal hydrostatic pressure, without causing any reduction of the survival rate of microorganisms. The method also permits the elimination of such a secondary effect that the subject microorganism undergoes proliferation during staining the same with a fluorescent dye. Thus, the present invention would contribute to the determination of the correct viable count in a wide variety of technical fields.
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申请公布号 |
US6358700(B2) |
申请公布日期 |
2002.03.19 |
申请号 |
US19980190168 |
申请日期 |
1998.11.12 |
申请人 |
JAPAN MARINE SCIENCE AND TECHNOLOGY CENTER |
发明人 |
FUMIYOSHI ABE;HORIKOSHI KOKI |
分类号 |
G01N33/48;C12Q1/04;C12Q1/06;C12R1/865;G01N1/30;G01N15/14;G01N33/483;(IPC1-7):C12Q1/04;C12N1/16;C12Q1/02 |
主分类号 |
G01N33/48 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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