A METHOD FOR SEQUENCING NUCLEIC ACID SEQUENCES WITHOUT CHAIN TERMINATION

摘要

A method is provided for determining the sequence of a nucleic acid sequence via incubation of a primer extended product of the nucleic acid sequence in separate pools of semi-random oligonucleotides of 5'(N)>n<A3', 5'(N)>n<C3', 5'(N)>n<G3', and 5'(N)>n<T3', wherein N is A, C, G or T and n is selected so that the oligonucleotides will optimally anneal to all complementary sequences.