DNA SEQUENCE AND A METHOD FOR THE IN VIVO MARKING AND THE ANALYSIS OF DNA/CHROMATIN IN CELLS

摘要

Disclosed is a method for the in vivo marking of DNA/chromatin structures in a cell. The inventive method is characterised in that a DNA sequence, preferably inserted into an expression vector, is expressed in the cell. Said sequence codes for fusion protein which comprises a histone protein and a fluorescent protein. The invention also relates to a method for the analysis of the DNA structure, chromatin structure, core structure, cell structure or cell dynamics, apoptosis, etc., whereby said method is based upon said in vivo marking. In a special embodiment of the inventive method, the fusion protein is linked to a his-tag . Histone proteins can thus be easily isolated.