发明名称 |
METHOD FOR PRODUCING DIVERSE LIBRARIES OF ENCODED POLYPEPTIDES |
摘要 |
tRNA analogues which comprise a tRNA, such as tRNAphe; an amino acid moiety which acts as an acceptor substrate, but not as a donor substrate, for ribosome-directed peptidyl transfer and, thus, is stably linked to the acceptor stem of the tRNA; and a reactive or activatible moiety near or within the anticodon stem loop of the tRNA that can medidate the covalent coupling of the tRNA analogue to mRNA. Also described are polypeptide-tRNA analogue-mRNA fusions; libraries of encoded polypeptides; methods of producing and screening the libraries; and target members and their uses.
|
申请公布号 |
US2002031762(A1) |
申请公布日期 |
2002.03.14 |
申请号 |
US19990291704 |
申请日期 |
1999.04.14 |
申请人 |
MERRYMAN CHARLES EVERETT;BARTEL DAVID P. |
发明人 |
MERRYMAN CHARLES EVERETT;BARTEL DAVID P. |
分类号 |
C12N15/10;C12N15/11;(IPC1-7):C12Q1/68;C07H21/02;C07H21/04;C12P21/04;C07K9/00 |
主分类号 |
C12N15/10 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|