| 摘要 |
<p>The invention relates to a cell line expressing mutant protein -human tissue-type- plasminogen activator, and the method for constructing it, and to the method for producing the mutant protein. The cell line of present invention has been deposited as Accession NO: CCTCC C200006. The method of constructing the cell line as follows: utilizing the DNA sequence of related gene having no important physiological function of short-arm of humanic chromosome on D, G group or the DNA sequence having homology with above DNA sequence as leading sequence having homology with above DNA sequence as leading sequence of aim gene; and constructing TNK-TPA transformant having specific gene vector; introducing TNK-TPA genne into nucleoli tissue target region D, G chromosome group of host cellHT1080 by the trasformant, obtaining cell line; and using the cell line to preparing TNK-TPA protein.</p> |
