发明名称 |
Methods of isolating genes encoding proteins of specific function and of screening for pharmaceutically active agents |
摘要 |
A method of isolating polynucleotides encoding polypeptides affecting an organization of a subcellular organelle or structure of interest is provided. The method comprises: (a) expressing within a plurality of cells an expression library including a plurality of expression constructs each encoding a polypeptide of interest; (b) highlighting the subcellular organelle or structure of interest of the plurality of cells; and (c) isolating a cell or cells of the plurality of cells in which a cellular distribution and/or level of the subcellular organelle or structure of interest is altered to thereby isolate polypeptides capable of affecting the organization of the subcellular organelle or structure of interest. |
申请公布号 |
AU8437301(A) |
申请公布日期 |
2002.03.13 |
申请号 |
AU20010084373 |
申请日期 |
2001.08.29 |
申请人 |
YEDA RESEARCH AND DEVELOPMENT CO..LTD. |
发明人 |
BENJAMIN GEIGER;ZVI KAM;ELI ZAMIR;ALEXANDER BERSHADSKY;MICHAEL SHTUTMAN;AVRI BEN-ZE'EV |
分类号 |
C12N5/10;C12N15/09;C12N15/10;C12Q1/02;G01N33/15;G01N33/50 |
主分类号 |
C12N5/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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