| 摘要 |
The present invention achieves convenient, rapid tests for three diseases, galactosemia, maple syrup urine disease, and phenylketonuria, which are inborn errors of metabolism. This invention is a testing method and a testing apparatus which use, at least as reaction reagents, respective dehydrogenases specific for D-galactose, branched chain amino acids including L-leucine, and L-phenylalanine, as assay target substances in a biological sample, a coenzyme, an electron mediator, and a tetrazolium salt, and which detect formazan, dependent on the concentration of the target substance and finally formed by an enzyme reaction and an oxidation-reduction reaction between the biological sample and the reaction reagents, by means of an optical detection method and/or an electrochemical detection method, thereby determining the target substances conveniently and rapidly. <IMAGE> |
