Novel type of transposon-based genetic marker

摘要

The present invention relates to the use of DNA primers homologous to MITE in a method for detecting polymorphisms of a nucleic acid sequence. The method comprises the steps of amplifying nucleic acid sequences using a first primer homologous to a MITE in combination with another primer homologous or not to a MITE, separating fragments of the nucleic acid sequences amplified, and analyzing the fragments obtained in relation to reference fragments obtained from amplification of a nucleic acid sequence with the primer homologous to MITE for determining polymorphism in the nucleic acid sequence. DNA primers homologous to MITE may also be used in genotyping, fingerprinting, mapping or cloning method in accordance with the invention.