ENZYME LIQUOR AND PROCESS FOR PRODUCING THE SAME, ENZYME PREPARATION, PROTEASE PREPARATIONS AND PROTEASE-PRODUCING BACTERIUM

摘要

An enzyme solution obtained by cultivating Bacillus subtilis M2-4 strain having highly heat-resistant peptidase activity such that the residual activity thereof after 1-hr heat treatment at 60 to 65 DEG C at pH 7 is substantially 100 %. An enzyme preparation can be obtained by separating the enzyme protein from the enzyme solution. Using such enzyme solution or enzyme preparation as an active component, a proteolytic enzyme preparation can be prepared. The enzyme solution, the enzyme preparation and the proteolytic enzyme preparation have both high heat-resistance and great potency of hydrolyzing proteins into low molecules.