发明名称 |
Mass spectrometric analysis of biopolymers |
摘要 |
The present invention makes use of unique tags of a specific biopolymer that can be exploited for determining the concentration the biopolymer in crude solutions. In preferred embodiments the biopolymer is either a protein or a polynucleotide. Particularly, the invention provides a method for the determination and quantitation of biomolecules in crude mixtures by way of a separation technique in combination with mass spectroscopy. In one general embodiment, a target biomolecule is selected for analysis and an analog thereof is generated. Peak area integration of the peptide pairs provides a direct measure for the amount of target protein in the crude solution. |
申请公布号 |
AU8506301(A) |
申请公布日期 |
2002.03.13 |
申请号 |
AU20010085063 |
申请日期 |
2001.08.17 |
申请人 |
GENENCOR INTERNATIONAL, INC. |
发明人 |
CHRISTIAN PAECH;SIGRID PAECH;DAVID A. ESTELL;GRANT C. GANSHAW |
分类号 |
C12P21/06;C12Q1/37;C12Q1/68;G01N33/00;G01N33/48;G01N33/50;G01N33/53;G01N33/68;G06F17/00;G06F19/00;H01J49/04 |
主分类号 |
C12P21/06 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|