| 摘要 |
<p>A method for determining a substrate wherein a substrate in a sample is determined by providing a biosensor having a structure in which electrodes are formed on the same plane by the use of electrically conductive materials, and reaction reagents including at least a dehydrogenase, a coenzyme, an electronic mediator and a tetrazolium salt are fixed on absorbent carriers and arranged on or above the electrodes, and detecting electrochemically a formazan produced by the supply of a sample; and a biosensor used therein. The method and the biosensor can be used for determining a substrate in a sample specifically, rapidly and with ease, without the need of a complicated pretreatment of the sample.</p> |
