METHOD FOR GENERATING RECOMBINANT DNA MOLECULES IN COMPLEX MIXTURES

摘要

Methods for generating libraries of recombinant DNA molecules by simultaneously recombining populations of DNA fragments in complex mixtures without cloning are provided. DNA derived from environmental samples, crude lysates, or partially enriched fractions of bacterial, or eukaryotic cells, or to cDNAs corresponding to RNA populations so derived, are fragmented and recombined. Optionally, the recombination is recursive. Methods for recovering and identifying recombinant DNA molecules encoding proteins with desirable characteristics are provided. Libraries of recombinant DNA molecules generated by the methods of the invention, as well as, cells incorporating such recombinant DNA molecules are provided.