EXPRESSION VECTOR CONTAINING UREA CYCLE ENZYME GENE, TRANSFORMANT THEREOF, AND USE OF TRANSFORMANT FOR PROTEIN OVER-EXPRESSION

摘要

The present invention relates to expression vectors containing in vivo urea cycle enzyme gene; transformants thereof; and the use of transformants for protein overexpression. During the overexpression of protein, if the animal cell transformed with expression vectors of the present invention is used, amounts of ammonia accumulation within cell culture medium will decrease and cell growth rate will increase, thus, it is advantageous to be capable of obtaining desired protein in high yields.