发明名称 |
PROCESS FOR THE PRODUCTION OF HUMAN CARTILAGE IMPLANTS BY MEANS OF CHONDROCYTES CULTIVATED IN VITRO |
摘要 |
The invention describes a process for the production of a human cartila ge implant from chondrocytes cultured in vitro, which come as close as possible to the original with respect to their biochemical composition and biomechanical properties. Up to 20% by vol. of human serum are used as medium addition in the process. The chondrocytes can be kept in monolayer culture until the 12th passage in order firstly to be re- differentiated under a reduced oxygen partial pressure and subsequently to be stimulated to form a three-dimensional cartilage tissue due to aggregation under an oxygen partial pressure of 21%.
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申请公布号 |
CA2356024(A1) |
申请公布日期 |
2002.02.28 |
申请号 |
CA20012356024 |
申请日期 |
2001.08.27 |
申请人 |
MERCK PATENT GESELLSCHAFT MIT BESCHRANKTER HAFTUNG |
发明人 |
MEENEN, NORBERT;GOPFERT, CHRISTIANE;ADAMIETZ, PETER;MEYER, JORG;JESCHKE, BRIGITTE |
分类号 |
A61K35/12;A61L27/00;A61L27/20;A61L27/38;A61L27/52;C12N5/077;(IPC1-7):A61L27/36;C12N5/06;C12N5/08;A61F2/30 |
主分类号 |
A61K35/12 |
代理机构 |
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地址 |
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