摘要 |
<p>Fluorescence of a thin, 100nm-thick layer (1a) of colour-tagged molecules is detected and measured on a glass substrate (2) to detect fluorescent efficiency quantitatively by relying on a lateral spot in the layer. To colour tag this layer, it is placed on the inner side of a channel (3) filled with a watery solution. The substrate and a channel rear wall (4) form the channel. Back reflection and back dispersion of stimulating light affect a lens (5) with interference.</p> |