| 摘要 |
Disclosed are an apparatus and method for two-dimensional electrophoretic separation and subsequent isolation of analytes of interest, particularly polypeptides. The apparatus includes a sample separation cavity comprising a first electrophoresis region for performing charge and/or sized-based electrophoresis in a first dimension, and a second electrophoresis region for performing electrophoresis in a second dimension, in a direction substantially perpendicular to the first dimension. The second region also contains a plurality of elongate separation channels substantially perpendicular to the first dimension, and these channels contain a removable solid phase effective to immobilize separated components upon activation. Non-immobilized components can then be removed, and immobilized components recovered, without loss of resolution. |
