| 摘要 |
This invention provides a method for determining the amount of a protein in a biological sample, which protein has the following characteristics: (1) selectively binds to a transmembrane isoform of a neuregulin protein and (2) induces nuclear translocation of a cytoplasmic domain of the transmembrane isoform of the neuregulin protein, which comprises: a) obtaining a biological sample from a subject; b) providing a ell comprising: (1) a first recombinant nucleic acid comprising a first DNA region encoding a transmembrane isoform of a neuregulin protein linked in frame to a second DNA region encoding a transcription factor wherein expression of the first recombinant nucleic acid in the cell produces a transmembrane isoform of a neuregulin protein-transcription factor fusion protein, and (2) a second recombinant nucleic acid comprising a promoter operatively linked to a reporter gene, wherein binding of the transcription factor portion of the fusion protein to the promoter activates expression of the reporter gene; c) contacting the biological sample with the cell; d) measuring reporter gene expression level in the cell; e) comparing the reporter gene expression level measured in step d) with a reporter gene expression level measured in multiple samples and multiple different known amounts of protein which selectively binds to a transmembrane isoform of a neuregulin protein, thereby determining the amount of a protein in a biological sample, which protein has the following characteristics: (1) selectively binds to a transmembrane isoform of a neuregulin protein and (2) induces nuclear translocation of a cytoplasmic domain of the transmembrane isoform of he neuregulin protein. This invention further provides methods for detecting other functional molecules, methods for treating diseases, including neurodegenerative diseases and cancer, compounds and pharmaceutical compositions, and cells used in the methods describes herein. |
