发明名称 |
POLYNUCLEOTIDE VACCINES EXPRESSING CODON OPTIMIZED HIV-1 NEF AND MODIFIED HIV-1 NEF |
摘要 |
Pharmaceutical compositions which comprise HIV Nef DNA vaccines are disclosed, along with the production and use of these DNA vaccines. The nef-based DNA vaccines of the invention are administered directly introduced into living vertebrate tissue, preferably humans, and express the HIV Nef protein or biologically relevant portions thereof, inducing a cellular immune response which specifically recognizes human immunodeficiency virus-1 (HIV-1). The DNA molecules which comprise the open reading frame of these DNA vaccines are synthetic DNA molecules encoding codon optimized HIV-1 Nef and derivatives of optimized HIV-1 Nef, including nef modifications comprising amino terminal leader peptides, removal of the amino terminal myristylation site, and/or modification of the Nef dileucine motif. These modifications may effect wild type characteristics of Nef, such as myristylation and down regulation of host CD4.
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申请公布号 |
WO0143693(A3) |
申请公布日期 |
2002.02.21 |
申请号 |
WO2000US34162 |
申请日期 |
2000.12.15 |
申请人 |
MERCK & CO., INC.;SHIVER, JOHN, W.;LIANG, XIAOPING;FU, TONG-MING |
发明人 |
SHIVER, JOHN, W.;LIANG, XIAOPING;FU, TONG-MING |
分类号 |
C12N15/09;A61K39/00;A61K39/39;A61P31/18;C07K14/16;(IPC1-7):C07H21/04;C12N15/00 |
主分类号 |
C12N15/09 |
代理机构 |
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