TRANSCRIPTION FACTOR TARGET GENE DISCOVERY

摘要

The ability to rapidly define transcription factor target genes allows for t he study of genetic cascades involved in development, physiology and disease. T he presently descibed invention outlines the combination of novel chromosomal immunoprecipitation and molecular biology technologies fot the high-throughp ut in vico discovery and characterization of both known and unknown transcripti on factor target genes. Through an application of solid phase support matrices and sequential chromosomal immunoprecipitation in combination with molecular cloning procedures this methodology allows for the rapid, stringent purification from cells and tissues of nucleotide sequences representing targets for regulation by transcription factors. Implementation of the technology described herein will result in the efficient simultaneous characterization of both regulatory element and coding sequence target gene information and will be valuable for assessing genetic hierarchies and developing therapeutics.