Probes and methods are provided for detection and analysis of nucleic acid sequences. The probes are single-labeled oligonucleotide probes whose fluorescence emission changes in response to probe-target hybridization and dissociation. The methods are for analyzing one or multiple nucleic acid loci using the probes. This invention further relates to the use of fluorescence changes in single-labeled probes for melting curve analysis, genotyping, and pathogen detection, and to methods for quantification of specific sequences in real-time monitoring of nucleic acid amplification.
申请公布号
WO0214555(A2)
申请公布日期
2002.02.21
申请号
WO2001US25231
申请日期
2001.08.10
申请人
UNIVERSITY OF UTAH RESEARCH FOUNDATION;IDAHO TECHNOLOGY, INC.