BARCODED SYNTHETIC LETHAL SCREENING TO IDENTIFY DRUG TARGETS

摘要

The present invention relates to methods of using synthetic lethal screening techniques to identify drug targets. The methods of the present invention use "barcoded" libraries of cells, where the library consists of a collection of different mutant clones, each mutant clone bearing a knock-out mutation of a different gene. Each mutant clone has a unique DNA identifier tag, or "barcode", to allow for quick and convenient identification of the clone and its mutation. The use of such a library allows for rapid, quantitative, sensitive and simple identification of genes which interact with a mutated target gene. So identified genes are promising targets for drug screening.