| 摘要 |
<p>The ability to rapidly define transcription factor target genes allows for the study of genetic cascades involved in development, physiology and disease. The presently descibed invention outlines the combination of novel chromosomal immunoprecipitation and molecular biology technologies fot the high-throughput in vico discovery and characterization of both known and unknown transcription factor target genes. Through an application of solid phase support matrices and sequential chromosomal immunoprecipitation in combination with molecular cloning procedures this methodology allows for the rapid, stringent purification from cells and tissues of nucleotide sequences representing targets for regulation by transcription factors. Implementation of the technology described herein will result in the efficient simultaneous characterization of both regulatory element and coding sequence target gene information and will be valuable for assessing genetic hierarchies and developing therapeutics.</p> |
