PYRUVATE:NADP+ OXIDOREDUCTASE AND USES THEREOF

摘要

Provided are polynucleotides encoding Pyruvate:NADP+ oxidoreductases (PNO) as well as methods for obtaining the same. Furthermore, vectors comprising said polynucleotides are described, wherein the polynucleotides are operatively linked to expression control sequences allowing the expression in prokaryotic and/or eukaryotic host cells. In addition, polypeptides encoded by said polynucleotides, antibodies to said polypeptides and methods for their production are provided. Further described are methods for increasing the acetyl CoA synthesis as well as methods for the production of fatty acids, carotenoids, isoprenoids, vitamins, lipids, wax esters, (poly)saccharides and/or polyhydroxyalkanoates, or its metabolism products, in particular, steroid hormones, prostaglandin, cholesterol, triacylglycerols, bile acids or ketone bodies, comprising the expression of the polynucleotide or polypeptide described herein in a host cell or plant cell, plant tissue or plant. Methods for the identification of compounds being capable of activating or inhibiting PNO are described as well. Further, a pharmaceutical composition comprising the afore-mentioned inhibiting compounds and antibodies is described. Furthermore, transgenic plants, plant tissues, and plant cells containing the above described polynucleotides and vectors are described as well as the use of the mentioned polynucleotides, vectors, polypeptides, antibodies, and/or compounds identified by the method of the invention in the production of acetyl CoA metabolism products, e.g., fatty acids, carotenoids, isoprenoids, vitamins, lipids, (poly)saccharides, wax esters, and/or polyhydroxyalkanoates, and/or its metabolism products, in particular, steroid hormones, prostaglandin, cholesterol, triacylglycerols, bile acids and/or ketone bodies and pharmaceutical compositions.