SYNTHETIC INSECTICIDAL TOXIN GENE FOR TRANSFORMATION OF BRASSICA PLANTS

摘要

PURPOSE: A synthetic insecticidal toxin gene for the transformation of Brassicae plants is provided, thereby effectively preventing plants from being damaged by diamondback moth, Plutella xylostella. CONSTITUTION: The synthetic insecticidal toxin gene for the transformation of Brassicae pants cryIAc has the nucleotide sequence of SEQ ID NO: 25. The synthetic cryIAc gene is prepared by the steps of: preparing a table showing the codon usage of cryIAc gene and Brassicae gene; preparing the revision strategy of cryIAc gene to have the nucleotide sequence similar to that of the Brassicae gene without change of the amino acid sequence; preparing 56 primers and carrying out the recursive PCR using 56 primers; and cloning the synthetic cryIAc gene and sequencing the synthetic cryIAc gene, in which the 56 primers are divided into three sets wherein the first primer set is from 1,344 to 1,854 base of the cryIAc gene, the second primer set is from 864 to 1,351 base of the cryIAc gene, and the third primer set is from 1 to 900 of the cryIAc gene.