发明名称 |
Verfahren zum Nachweis von Mutationen in Nucleotidsequenzen |
摘要 |
The invention relates to a method for simultaneously detecting mutations in different nucleotide sequences and for determining the transcription rate of mutated and non-mutated nucleotide sequences. The inventive method comprises the following steps: hybridizing single-stranded sample nucleotide sequences to single-stranded reference nucleotide sequences, fixating, before or during hybridization, single-stranded reference nucleotide sequences or single-stranded sample nucleotide sequences, or fixating, after or during hybridization, heteroduplices from reference and sample nucleotide sequences on an electronically addressable surface, incubating them with a substrate that recognizes heteroduplex mismatches, and detecting the substrate bonds.
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申请公布号 |
DE10038237(A1) |
申请公布日期 |
2002.02.14 |
申请号 |
DE20001038237 |
申请日期 |
2000.08.04 |
申请人 |
AVENTIS RESEARCH & TECHNOLOGIES GMBH & CO KG |
发明人 |
KAPPEL, ANDREAS;PIGNOT, MARC;POLAKOWSKI, THOMAS;WINDHAB, NORBERT |
分类号 |
G01N33/53;B01J19/00;C07H21/00;C07K14/195;C07K14/245;C12N9/00;C12N15/09;C12N15/52;C12Q;C12Q1/25;C12Q1/34;C12Q1/68;G01N33/566;(IPC1-7):C12Q1/68 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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