THE USE AND EVALUATION OF A [2+2] PHOTOCYCLOADDITION IN IMMOBILIZATION OF OLIGONUCLEOTIDES ON A THREE-DIMENSIONAL HYDROGEL MATRIX

摘要

[0064] [2+2]photo-attachable functional groups were incorporated in polyacrylamide based hydrogels and synthetic oligonucleotide probes. The probes were photochemically attached by covalent bonding to the three dimensional surface of a hydrogel. The resultant hydrogel microarrays were used for detection of specific target oligonulcleotides, including mRNA and DNA, as suitable for expression and single nucleotide polymorphism type microarray analyses.