ENHANCEMENT OF INDUSTRIAL PRODUCTION BY INCREASING SUBSTRATETRANSPORT

摘要

A method for enhancing a host cell's biosynthetic production of a desired compound derived from an at least partially intracellular pathway of said ho st cell is described. Such method comprises selecting a host cell that has an a t least partially intracellular synthetic pathway which utilizes an extracellular substrate; increasing the transport of said extracellular substrate into said host cell while maintaining the integrity of the host cell, culturing the host cell to produce said extracellular substrate; and producing the desired compound. The transport of the substrate is increased by transforming into the host cell DNA encoding for one or more enzymes transporting the substrate into the host cell.