发明名称 Method for cellular lysis of prokaryotes and eukaryotes or simultaneous cellularlysis or prokaryotes and eukaryotes
摘要 A method for lysing prokaryotic or eukaryotic cells, or for simultaneous lysis of both, which includes at least three of the following: a mass of active, small-diameter beads corresponding to 50% or less than a mass of active, larger-diameter beads, and/or a total mass of lysing beads (consisting of a single type of bead or a mixture of smaller and larger beads) corresponding to between 50 and 100% of the total mass of the processed biological sample, and/or a lysis time of from 10 to 20 minutes, and/or seven or less non-lysing glass beads to drive the movement of the lysing beads, and/or from five to fifteen non-lysing iron beads to drive the movement of the lysing beads, depending on whether sonication, mechanical vortex centrifugation or magnetic vortex centrifugation is used.
申请公布号 AU7991301(A) 申请公布日期 2002.02.13
申请号 AU20010079913 申请日期 2001.07.26
申请人 BIOMERIEUX SA 发明人 PHILIPPE CLEUZIAT;SANDRA INCARDONA;CORINNE JAY
分类号 C12N1/06;C12M1/33;C12M3/08 主分类号 C12N1/06
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