Manipulation of genes of the mevalonate and isoprenoid pathways to create novel traits in transgenic organisms

摘要

The present invention provides a method of providing a cell with an inserted polynucleotide sequence encoding one or more products of interest comprising the steps of: providing a plurality of target cells having an identified pseudogene site therein; providing an isolated promoterless polynucleotide comprising polynucleotide sequences of said pseudogene site flanking at least one coding sequence of interest; introducing said promoterless polynucleotide into a plurality of said target cells; and selecting at least one target cell which contains the coding sequence of interest inserted into said pseudogene site, wherein said coding sequence of interest is expressed under the control of regulatory elements of said pseudogene site. The invention also relates to a transformed cell produced by the method of the invention, its descendant cell, and uses of such cell.