| 摘要 |
PROBLEM TO BE SOLVED: To provide a material and a method for detection of a nucleic acid target sequence. SOLUTION: The invention employs an unlabeled signal primer comprising a 5'-side adapter sequence for detection of the nucleic acid target sequence. The detection system further comprises a reporter probe, the 3'-side end of which hybridizes to the complementary chain of the 5'-side adapter sequence of the signal primer to produce a 5'-side overhang. Polymerase is used to fill in the overhang and synthesize the complement of the 5'-side overhang of the reporter probe. Synthesis of the reporter probe complementary chain is detected, either directly or indirectly, as an indication of the presence of the target. |
