| 摘要 |
PURPOSE: A method for preparing bone transplanter is provided to obtain an excellent bone transplantation effect without occurring an inflammation and an immune rejection reaction after transplanting. CONSTITUTION: In the method for preparing bone transplanter, allogenic bone is collected, and soft tissue is removed from the bone. The bone is pulverized, washed, and classified into bone pieces and bone powders. The split bone pieces or bone powders are washed and defatted using ethanol and ether, respectively. The bone is dried at room temperature, and demineralized using hydrochloric acid. The demineralized bone is washed with saline solution or phosphate buffered saline, and further washed with ethanol and ether. The washed bone is dried at room temperature and lyophilized. The lyophilized bone is further classified by sizes to obtain bone transplanter.
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