发明名称 |
SCREENING AND USE OF AGENTS WHICH BLOCK OR ACTIVATE INTEIN SPLICING UTILIZING NATURAL OR HOMOLOGOUS EXTEINS |
摘要 |
Positive genetic selection systems are provided with methods for screening agents that control intein-mediated protein splicing innative host proteins - exteins - or in homologous exteins. Agents inhibiting or activating protein splicing are detected by eithercell viability or cell death where a positive genetic selection system uses a cell comprising, as in Figure 9, a first gene encoding a wild type enzyme, or a drug-resistant enzyme, and a second gene encoding a dominant cytotoxic form of the enzyme comprising an intein, or a drug-susceptible cytotoxic form of the enzyme comprising an intein. Non-lethal selection systems and assays are further provided as well as exemplary mycobacterial gyrase- and helicase-associated inteins. Also provided are controllable inteins and methods for in vivo control of drug delivery and methods for in vivo control of protein expression wherein protein splicing is modulated.
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申请公布号 |
WO0132831(A3) |
申请公布日期 |
2002.02.07 |
申请号 |
WO2000US29596 |
申请日期 |
2000.10.27 |
申请人 |
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发明人 |
PERLER, FRANCINE, B.;ADAM, ERIC, E. |
分类号 |
G01N33/50;A61K45/00;A61P31/04;C12N1/21;C12N9/90;C12N15/09;C12N15/10;C12N15/62;C12N15/63;C12N15/64;C12Q1/00;C12Q1/02;C12Q1/04;C12Q1/37;C12Q1/48;C12Q1/68;G01N33/15;G01N33/53;G01N33/566;(IPC1-7):C12N15/62;C12N15/70;C12N15/52;C12Q1/18;C12Q1/25;C07K19/00;A61K38/16 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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