发明名称 |
Sets of oligonucleotide-binding e-tag probes |
摘要 |
Probe sets for the multiplexed detection of known, selected nucleotide target sequences are provided. Detection involves the release of identifying tags as a consequence of target recognition. The probe sets include electrophoretic tag probes or "e-tag probes", comprising a detection region and a mobility-defining region called the mobility modifier, both linked to a target-binding moiety. The target-binding moiety of the e-tag probes hybridizes to complementary target sequences followed by nuclease cleavage of the e-tag probes and release of detectable e-tags or e-tag reporters. The mixture is exposed to a capture agent which binds uncleaved and/or partially cleaved e-tag probes, followed by electrophoretic separation. In a multiplexed assay, different released e-tag reporters may be separated and detected providing for target identification.
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申请公布号 |
US2002015954(A1) |
申请公布日期 |
2002.02.07 |
申请号 |
US20010825246 |
申请日期 |
2001.04.02 |
申请人 |
SINGH SHARAT;MATRAY TRACY;CHENNA AHMED |
发明人 |
SINGH SHARAT;MATRAY TRACY;CHENNA AHMED |
分类号 |
C12N15/09;C07B61/00;C07H19/06;C07H19/10;C07H21/00;C12Q1/68;(IPC1-7):C12Q1/68;C07H21/04 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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