SYNTHETIC AND RECOMBINANT SUBSTRATES FOR THE DETECTION OF THE VON WILLEBRAND FACTOR-CLEAVING PROTEASE
摘要
A method of detecting von Willebrand Factor-cleaving protease in a test sample is disclosed. In one embodiment, the method comprises the steps of (a) obtaining a test bodily fluid sample; (b) exposing the test sample to a monomeric von Willebrand Factor fragment, wherein the fragment comprises amino acids 842 and 843, wherein cleavage of the fragment will occur proportional to the amount of protease in the sample; and (c) comparing the cleavage products to a standard curve and determining the amount of von Willebrand Factor-cleaving protease in the test sample.
申请公布号
WO0210437(A2)
申请公布日期
2002.02.07
申请号
WO2001US23784
申请日期
2001.07.27
申请人
BLOOD CENTER RESEARCH FOUNDATION;MCW RESEARCH FOUNDATION, INC.;MONTGOMERY, ROBERT, R.;RAIFE, THOMAS, J.;HABERICHTER, SANDRA, L.
发明人
MONTGOMERY, ROBERT, R.;RAIFE, THOMAS, J.;HABERICHTER, SANDRA, L.