A PLATELET AGGREGATION INHIBITOR DERIVED FROM SNAKE VENOM AND PROCESS FOR PREPARING THE SAME

摘要

The present invention relates to Sal-C protein derived from snake venom of Agkistrodon halys brevicaudus with platelet aggregation inhibitory activity, cDNA thereof and process for preparing the same. The protein of the present invention is prepared by the process comprising the step of collecting snake venom of Agkistrodon halys brevicaudus and purifying Sal-C protein from the venom through anion exchange chromatography, gel filtration and HPLC reverse chromatography. The protein is 28kDa protein comprising disintegrin-like domain and C-terminal domain. The Sal-C protein of the present invention effectively represses platelet aggregation caused by ADP mediated by the platelet surface glycoprotein as well as collagen and therefore can be widely applied to the effective materials of agents for treating thrombosis caused by platelet aggregation.