The present invention provides novel compositions and methods for suppressing the expression of a targeted gene using mRNA-cDNA duplexes. The invention further provides novel methods and compositions for generating amplified mRNA-cDNA hybrids, whose quantity is high enough to be used for the invention's gene silencing transfection. This improved RNA-polymerase chain reaction method uses thermocycling steps of promoter-linked double-stranded cDNA or RNA synthesis, <i>in vitro</i> transcription and then reverse transcription to amplify the amount of mRNA-cDNA hybrids up to two thousand folds within one round of the above procedure.
申请公布号
WO0210374(A2)
申请公布日期
2002.02.07
申请号
WO2001US24412
申请日期
2001.08.02
申请人
UNIVERSITY OF SOUTHERN CALIFORNIA;LIN, SHI-LUNG;CHUONG, CHENG-MING;WIDELITZ, RANDALL, B.
发明人
LIN, SHI-LUNG;CHUONG, CHENG-MING;WIDELITZ, RANDALL, B.