METHOD FOR DETERMINING A PARTIAL NUCLEOTIDE SEQUENCE OF A NUCLEIC ACID MOLECULE

摘要

The invention relates to a method for determining a partial nucleotide sequence of a nucleic acid molecule, comprising the following steps: (a) incubating a primer which hybridizes to the nucleic acid molecule with multiple copies of the nucleic acid molecule in the presence of a polymerase and a mixture of desoxyribonucleotides; (b) determining the molecular masses of nucleic acid fragments generated by the elongation of the primer in step (a) which succeed each other in size, by means of mass spectrometry; and (c) determining the nucleotide sequence by determining the growth in molecular mass between the nucleic acid fragments which succeed each other in size.