发明名称 |
DOWN-REGULATION OF SINGLE GENES AND SIMULTANEOUS DOWN-REGULATION OF MULTIPLE GENES BY NUCLEAR LOCALIZATION OF RNA TRANSCRIPTS |
摘要 |
A method is provided for gene suppression in eukaryotes by transformation with a recombinant construct containing a promoter, at least one antisense and/or sense nucleotide sequence for the gene(s) to be suppressed, wherein the nucleus-to-cytoplasm transport of the transcription products of the construct is inhibited. In one embodiment, nucleus-to-cytoplasm transport is inhibited by the absence of a normal 3' UTR. The construct can optionally include at least one self-cleaving ribozyme. The construct can also optionally include sense and/or antisense sequences to multiple genes that are to be simultaneously down-regulated using a single promoter. Also disclosed are vectors, plants, animals, seeds, gametes, and embryos containing the recombinant constructs. |
申请公布号 |
WO0210365(A2) |
申请公布日期 |
2002.02.07 |
申请号 |
WO2001US24355 |
申请日期 |
2001.08.02 |
申请人 |
THE BOARD OF REGENTS OF THE UNIVERSITY OF NEBRASKA |
发明人 |
CLEMENTE, TOM, E.;BUHR, TONY, L.;STASWICK, PAUL, E. |
分类号 |
C12N15/113;C12N15/82 |
主分类号 |
C12N15/113 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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