METHOD FOR COLLECTING AND PURIFYING RECOMBINANT ANTIGEN THAT PROTECTS PIG FROM ERYSIPELOTHRIX RHUSIOPATHIAE

摘要

PROBLEM TO BE SOLVED: To efficiently and industrially collecting and purifying an antigen that protects a pig from Erysipelothrix rhusiopathiae and is usable for producing Erysipelothrix rhusiopathiae vaccine. SOLUTION: This method comprises the steps of culturing Brevibacillus choshinensis transformed with a gene encoding 46.5 KPA protein having an antigenic activity of Erysipelothrix rhusiopathiae (46.5 kDa protective protein), filtrating the obtained culture broth using a precise membrane filter to collect an antigen protein and cells in a condensed state, washing the antigen protein and cells with an alkaline buffer to solubilize only the antigen protein, filtrating the obtained mixture, and further applying the anion exchange chromatography to the obtained filtrate fraction for purification.