Computer method and system for correlating known characteristics of biomolecules to molecular tags with unique molecular weights that are associated with the biomolecule

摘要

A method in a computer system for correlating known characteristics of biomolecules such as nucleic acid molecules to molecular tags with unique molecular weights that have been associated with the biomolecules or portions thereof comprises: receiving a mapping of each known characteristic of the biomolecules to the corresponding molecular weight of the molecular tag associated with the characteristic; receiving an indication of the molecular weights detected when analyzing the tags that have been dissociated from the biomolecules or portions thereof, wherein the tags are separated based on a determined characteristic of the associated biomolecules or portions thereof other than the sequences of the biomolecules before dissociation of the tag from the biomolecules and for each molecular weight detected: determining based on the received mapping the known characteristic of the biomolecule corresponding to the detected molecular weight; and indicating that the biomolecule has the determine characteristic. A computer system for known correlating characteristics of sample biomolecules to molecular tags with unique molecular weights that have been associated with the sample biomolecules or portions thereof comprises: a molecular tag definition table indicating the molecular weight of each molecular tag; a defined run component for generating a mapping each sample biomolecule to a molecular tag; a molecular weight collection component for receiving an indication of the molecular weights that have been associate with the sample biomolecules or portions thereof separated based on a determined characteristic of the associated biomolecules or portions thereof other than the sequences of the biomolecules and subsequently dissociated from the biomolecules or portions thereof and a correlation component that determines based on the received mapping, the known characteristic of the biomolecule corresponding to the detected molecular weight and indicates that the biomolecule has the determine characteristic.