| 摘要 |
The invention concerns a method for cellular lysis of prokaryotes or eukaryotes or simultaneous cellular lysis of prokaryotes and eukaryotes. It consists in adapting at least the following three parameters: a percentage by mass of active beads with small diameter relative to active beads with large diameter not more than 50 %, and/or a total mass of lysing beads, comprising or not a mixture of small diameter beads and/or large diameter beads, ranging between 50 and 100 % relative to the total mass of the treated biological sample, and/or a lysis duration ranging between 10 and 20 minutes, and/or a number of non-lysing glass beads but driving lysing beads less than seven (7), and/or a number of non-lysing iron beads but driving lysing beads ranging between five (5) and fifteen (15), based on the sonication, mechanical vortex, or magnetic vortex technique. The invention is in particular applicable in the field of cellular lysis.
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