| 发明名称 |
High temperature reverse transcription using mutant DNA polymerases |
| 摘要 |
The present invention relates to improved reverse transcription methods using a modified thermostable DNA polymerases, particularly in a magnesium ion buffer. These methods are particularly useful in combined reverse-transcription/amplification reactions.
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| 申请公布号 |
US2002012970(A1) |
申请公布日期 |
2002.01.31 |
| 申请号 |
US20010823649 |
申请日期 |
2001.03.30 |
| 申请人 |
SMITH EDWARD SOH;ELFSTROM CARITA MARIA;GELFAND DAVID HARROW;HIGUCHI RUSSELL GENE;MYERS THOMAS WILLIAM;SCHONBRUNNER NANCY JENEANE;WANG ALICE MING |
发明人 |
SMITH EDWARD SOH;ELFSTROM CARITA MARIA;GELFAND DAVID HARROW;HIGUCHI RUSSELL GENE;MYERS THOMAS WILLIAM;SCHONBRUNNER NANCY JENEANE;WANG ALICE MING |
| 分类号 |
C12N15/09;C12N9/12;C12Q1/68;(IPC1-7):C12P19/34 |
主分类号 |
C12N15/09 |
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