| 摘要 |
A method of producing mature somatic embryos (especially of conifers) to provide unexpected yield advantages comprising the steps of: (1) placing explants of the immature embryos on and/or in an initiation medium (the medium itself being part of the invention) or on a nurse culture itself on or in the initiation medium, (2) allowing the initiation to take place, and (3) (whether after optional storage and maintenance or not) maturing the initiated embryos on an appropriate maturation medium, wherein: (a) the initation and maturation medium may be the same or different, and wherein (b) (i) at least the initiating medium contains ABA and at least one amino acid, or (b) (ii) at least the initiating medium contains at least one amino acid and no ABA, and (c) at least the maturation medium contains ABA and at least one amino acid.
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