PRODUCTION OF LYSOSOMAL ENZYMES IN PLANTS BY TRANSIENT EXPRESSION
摘要
The invention relates to the production of enzymatically active recombinant human and animal lysosomal enzymes involving construction and expression of recombinant expression constructs comprising coding sequences of human or animal lysosomal enzymes in a plant expression system. The plant expression system provides for post-translational modification and processing to produce a recombinant gene product exhibiting enzymatic activity. The invention is demonstrated by working examples in which transgenic tobacco plants express recombinant expression constructs comprising human glucocerebrosidase nucleotide sequences. The invention is also demonstrated by working examples in which transfected tobacco plants express recombinant viral expression constructs comprising human alpha glactosidase nucleotide sequences. The recombinant lysosomal enzymes produced in accordance with the invention may be used for a variety or purposes, including but not limited to enzyme replacement therapy for the therapeutic treatment of human and animal lysosomal storages diseases.
申请公布号
WO0208404(A2)
申请公布日期
2002.01.31
申请号
WO2001US24111
申请日期
2001.07.26
申请人
LARGE SCALE BIOLOGY CORPORATION;GARGER, STEPHEN, J.;TURPEN, THOMAS, H.;KUMAGAI, MONTO, H.
发明人
GARGER, STEPHEN, J.;TURPEN, THOMAS, H.;KUMAGAI, MONTO, H.